Octagon group release 4-Aco-DMT synthesis

Here are the steps required to synthesize 4-Aco-DMT:
Materials needed:

  • Psilocin
  • Acetic anhydride
  • Hydrochloric acid
  • Sodium carbonate
  • Methanol
  • Ethanol
  • Sodium hydroxide
  • Ether
  • Water
  • Separatory funnel
  • Round bottom flask
  • Heating mantle
  • Stirring bar
  • Glassware (beakers, flasks, pipettes, etc.)
  • Safety equipment (gloves, goggles, lab coat, etc.)

Step 1: Preparation of Psilocin

  • Grind magic mushrooms into a fine powder
  • Soak the powder in methanol or ethanol for several hours
  • Filter the solution through a filter paper or a Buchner funnel
  • Evaporate the solvent using a rotary evaporator or a heat source, such as a heating mantle, to obtain a crude extract of psilocin

Step 2: Acetylation

  • Dissolve the crude extract of psilocin in a small volume of acetic anhydride and add a few drops of concentrated hydrochloric acid as a catalyst
  • Heat the mixture under gentle reflux for several hours, stirring occasionally
  • After the reaction is complete, allow the mixture to cool to room temperature and then pour it into a large volume of ice-cold water
  • The resulting precipitate should be filtered and washed several times with water to remove any impurities
  • The product can be further purified using column chromatography or recrystallization techniques

Step 3: Deacetylation

  • Dissolve the purified N-acetylpsilocin in a solution of sodium carbonate and water
  • Add a few drops of concentrated hydrochloric acid to the solution to adjust the pH to acidic
  • Extract the solution with ether several times to remove any impurities
  • Evaporate the ether under vacuum to obtain the freebase form of 4-Aco-DMT
  • Dissolve the freebase in a solution of sodium hydroxide and water
  • Extract the solution with ether several times to remove any impurities
  • Evaporate the ether under vacuum to obtain the hydrochloride salt form of 4-Aco-DMT

Step 4: Final Purification

  • The resulting 4-Aco-DMT hydrochloride salt can be further purified using recrystallization techniques or column chromatography to obtain a pure and potent form of the substance.

To begin, you shall need the following lab equipment and chemicals:

  • A 100 mL round-bottom flask
  • A stir bar
  • A reflux condenser
  • A heating mantle
  • Methanol (100 mL)
  • Hydrochloric acid (37%, 3 mL)
  • 4-AcO-DET (10 g)
  • Sodium cyanoborohydride (10 g)
  • Sodium hydroxide (10 g)
  • Diethyl ether
  • Anhydrous magnesium sulfate (MgSO4)

And now, onto the synthesis itself:

  1. First, dissolve 4-AcO-DET (10 g) in methanol (100 mL) in a 100 mL round-bottom flask. Add hydrochloric acid (37%, 3 mL) and stir the solution.
  2. Next, slowly add sodium cyanoborohydride (10 g) to the solution while stirring. The reaction mixture will turn yellow-brown and a white precipitate will form.
  3. Reflux the mixture for 24 hours with a reflux condenser and heating mantle. Stir the mixture occasionally.
  4. After 24 hours, let the mixture cool to room temperature and then carefully add 10 g of sodium hydroxide to the flask. Stir the mixture until all of the sodium hydroxide dissolves.
  5. Extract the mixture with diethyl ether (3 x 50 mL). Combine the ether layers and dry over anhydrous magnesium sulfate (MgSO4).
  6. Filter off the drying agent and evaporate the ether under reduced pressure to obtain a yellow-brown oil.
  7. Dissolve the crude product in a small amount of methanol and then add diethyl ether until the product precipitates out.
  8. Collect the product by filtration, wash it with diethyl ether, and then dry it under vacuum to yield pure 4-Aco-DMT.
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